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The expression levels of key proteins were analyzed by Western blot, and apoptosis was assessed by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and Annexin V/Propidium iodide (PI) staining. The mitochondrial function was assessed using flow cytometry. Nrf2 and protein interaction were assessed by immunoprecipitation. In vitro, BAF-treatment protected against oxidative stress-induced apoptosis, maintained mitochondrial function and improved cell survival. Moreover, BAF treatment was accompanied by restoration of the expression and activity of HIF-1α and Nrf2, and decreased the expression of Beclin-1. In vivo, BAF-treatment significantly reduced the release of lactate dehydrogenase and attenuated renal histopathological injury. Moreover, BAF treatment also decreased oxidative stress and improved cell survival. Further, BAF treatment induced the nuclear accumulation of Nrf2. These changes were accompanied by increases in the nuclear translocation of Nrf2 and protein expression of Nrf2 target genes. These results suggest that BAF protects the kidney against hypoxic injury through the HIF-1α/Nrf2/ARE pathway.Q: How to change the position of an individual Item in a CustomRow in nvd3 The NVD3 code I am working on is attached below. Is it possible to move the green bar under the red one? The only way I can get it to come under is to manually add negative margin to the nvd3 object. A: It sounds like you are looking for the option property on the BarSeries element. Its value is the x coordinate of What's New in the Adobe Photoshop CS6? Q: Topological space $X$, a subset $A\subseteq X$, and the collection $L$ of all open subsets of $X$ containing $A$. Show $L$ is a topological base for $X$. I am currently studying Kelley's General Topology, Second Edition, and am working on the exercise of page 157: Let $X$ be a topological space, let $A\subseteq X$, and let $L$ be the collection of all open subsets of $X$ containing $A$. Show that $L$ is a topological base for $X$. I am not familiar with this type of question, and I have been searching for answers for quite some time, but I'm unable to find anything that even comes close to what I am trying to understand. Since this exercise makes no assumptions about $X$ or $A$, I think it may be helpful to make a statement of the question using a very simple set $X$, and a simple set $A$, as I'm trying to determine how to best approach it. The following image is in reference to how I am approaching this question so far: In particular, I am unsure about the correct approach for determining whether $L$ is a topological base for $X$, and how to define the elements of $L$. I understand the basics of points and neighborhoods, and the idea of the topology of $X$, but I am having a hard time determining how to use this to solve this question. If you've managed to understand what I'm trying to ask, could you please try to explain what I am missing? A: We need to show that the topology it generates is the given topology on $X$. Let $x \in X$ and let $\mathscr{B}$ be a basis for the given topology on $X$. Then $\{x\} \cup A \cup \mathscr{B}$ is a base for the topology generated by $L$, as for every $B \in L$, there exists an open $U \subset X$ such that $x \in U, B \cap U \in \mathscr{B}$. Here is an attempt to help you understand the method of the proof: Each point $x \in X$ generates a trivial sub-basis for the System Requirements: Apple TV® 3, Apple TV 4K™, Apple TV HD™, Apple TV 2nd Generation, Apple TV 4th Generation, and the latest generation of Apple TV set-top boxes running tvOS 10.2 or later. 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